Shared Resources

The goal of this webpage -- and our Instrumental e-newsletter -- is to connect you with the services and expertise embedded within the Rogel Shared Resources (SRs).

Once again, we have several things to share including new instrumentation available within several of our facilities, exciting training opportunities, and a new recurring section to introduce you to the experts within the Cancer Data Science SR.

We hope you find these new offerings valuable assets to your ongoing research projects.

Epigenetics and Epigenomics SR (EE-SR)

The BRCF Epigenomics Core is not new to the institution, but it is new to the list of Rogel-supported SRs, having been approved as the developing Epigenetics and Epigenomics SR (EE-SR) with our most recent Cancer Center Support Grant renewal.

The EE-SR is ably led by faculty Director, Dana Dolinoy, Ph.D., and Managing Director, Claudia Lalancette, Ph.D., and provides resources and services for the analysis of gene regulation in both genome-wide (epigenomics) and locus-specific (epigenetics) manners, with a focus on cytosine modifications, chromatin immunoprecipitation, and chromatin accessibility/conformation.

For more information or to schedule services, please visit the EE-SR website.

Transgenic Animal Models SR Offers Training Class

The Transgenic Animal Models SR is offering a two-week hands-on training session from April 15-26. Participants will report daily to the Mouse ES Cell Laboratory (2578 MSRB II) while experts from the SR will provide instructions on how to design, build, and validate CRISPR/Cas9 reagents in mouse ES cells.

Trainees will learn how to work with mouse embryonic stem cells in culture, to clone CRISPR/Cas9 reagents in plasmids, and to purify and analyze DNA from cultured cells while also learning fundamental genetic principles for genetic engineering including:

• planning CRISPR/Cas9 genome editing workflows
• fundamental principles of gene targeting design
• how to apply Cre recombinase technology to conditional genes (“floxed genes”)

In addition, trainees will be introduced to the power of genetically engineered mice to model human disease and learn how to access resources from the International Knockout Mouse Consortium project to knockout every protein coding gene in the mouse. After attending, trainees will be able to design experiments to create genetic modifications and produce them in mouse ES cells and in mice with CRISPR/Cas9 reagents.

To reserve a place in this two-week class, please login to MiCORES and navigate to Transgenic Core:

• click on the Request Services tab
• click on the Training Classes folder
• submit a request for CRISPR/Cas9 ES Cell Training Class

Places are filled on a first-come first-serve basis. There is a $2499 fee to defray costs. Download a sample syllabus here.

Cancer Quantitative Data Sciences Symposium

In collaboration with the Cancer Data Science SR, the Rogel Cancer Center presents the 2024 Annual Symposium for Cancer Quantitative Data Sciences. This event, held May 16 at the School of Public Health, will include remarks from three keynote speakers centered around the topic of AI in Cancer Research: Predicting the Future.

Questions? Please contact Emily Klimas: [email protected]. Please register in advance.

Flow Cytometry adds imaging cell sorter

The Flow Cytometry SR has added a BD FACSDiscover S8 Imaging Cell Sorter to its BSRB, Room A681 location. This spectral flow cytometer sorter expands the power of cell analysis and sorting by combining spectral flow cytometry with real-time spatial and morphological insights. Reserve time to enhance your spectral flow cytometry via MiCORES.

Cell and Tissue Imaging SR to expand NCRC location

We are excited to announce the Cell and Tissue Imaging SR, also known as the BRCF Microscopy Core, is expanding its NCRC site. Located on the ground floor of Building 20, and currently home to a Nikon A1Si laser scanning confocal microscope, this space will soon house a Yokogawa spinning disk confocal microscope, ideal for imaging live samples.

Please pardon our dust as we reconfigure the space to make it suitable for handling BSL2 samples, which entails adding a tissue culture room containing a sink, eyewash, incubator, and biosafety cabinet. Additionally, there will be image analysis computers available, including an Imaris workstation, and the SR will be staffed approximately 20 hours per week. We anticipate that construction will be complete and the new microscope installed in late spring / early summer. Please stay tuned for more details, dates for an open house, and further expansion plans slated for the future.

Structure and drug screening

The Structure and Drug Screening SR, through the Center for Chemical Genomics (CCG), is now offering hERG channel testing using SyncroPatch, a highly innovative high-throughput technology for measuring ion channel activity. Visit the CCG website for more information on the importance of hERG screening in early-phase drug discovery projects.

Gen Li, Ph.D.
Associate Professor, Biostatistics
Assistant Director, Cancer Data Science SR

Dr. Li joined the Cancer Data Sciences SR three years ago and was recently named Assistant Director to lead in the areas of bioinformatics and laboratory sciences.

What is your area of expertise?
Multi-omics data integration, high-throughput data analysis, microbiome data analysis

What types of analyses do you run most frequently?
Association analysis, differential analysis, dimension reduction, graphical models

What is your favorite place in Ann Arbor?
Gallup park and Huron Hills Golf Course

Name a food you could eat every day.
Chinese food